3-Hydroxyproline content of normal urine.

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3-Hydroxyproline content of normal urine.

Values for total 3-hydroxyproline and 4-hydroxyproline were obtained from 24-h urine specimens of 18 healthy human subjects of both sexes, whose ages ranged from the first to the sixth decade in age. Urinary 3-hydroxyproline levels, not earlier described to our knowledge, were determined by an isotope-dilution method requiring considerable purification and utilizing the amino acid analyzer for ...

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The Calcium and Magnesium Content of Normal Urine

Considerable difference of opinion exists at present concerning the relative amounts of calcium and magnesium excreted in normal urine. Hammarstenl states that of a daily urinary excretion of somewhat more than 1 gm., two-thirds is magnesium and one-third calcium. Bertram in a 3 day period found 0.268 gm. of MgO and 0.167 gm. of CaO; RenvalJ3 and Long and Gephart,4 on the contrary, report figur...

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Studies on 3-methoxyproline and 3-hydroxyproline.

The cis and trans racemates of synthetic 3-hydroxyproline and of synthetic 3-methoxyproline were interconverted by an epimerization reaction at carbon atom 2. The interconversions are proof of the diastereomeric nature of the synthetic isomers. Assignments of cis and trans configuration were made from nuclear magnetic resonance studies. Synthetic cisand trans-3-hydroxy-DL-proline were partially...

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Effect of Ultrasound Therapy on the Hydroxyproline Content in Experimental Tendon Injuries in Horses.

Objective-To determine the effect of ultrasound therapy on hydroxyproline (HP) content in superficial digital flexor tendon (SDFT) in horses.Design - Experimental in vivo study.Animals - Eight adult castrated horses.Procedure- The mediocaudal surface of right metatarsal bone in was exposed and after complete separation of SDFT, it was splitted (striking 15 times) in full thickness in longitudin...

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A method for serum C1q based on its hydroxyproline content.

The radial immunodiffusion assay overestimates the C1q in serum. Here we describe a convenient, accurate procedure for measuring C1q in 250 microL of dialyzed serum. This method is based on our previous findings that all C1q in serum precipitates with the euglobulin fraction and that all other serum proteins containing hydroxyproline are excluded from this fraction. Because C1q is 4.3% hydroxyp...

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ژورنال

عنوان ژورنال: Journal of Clinical Investigation

سال: 1978

ISSN: 0021-9738

DOI: 10.1172/jci109068